Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor.
Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55793 | G protein alpha-s-CFP | G protein alpha-s internally tagged with ECFP and EE epitope |
| 55794 | Beta-2-adrenergic receptor-CFP | Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus |
| 55795 | Dyn1(K44A)-mRFP | Dominant negative Dynamin 1 mutant tagged with mRFP |
| 66083 | G protein alpha-s-GFP | G protein alpha-s internally tagged with EGFP and EE epitope |
| 66084 | G protein alpha-s-mCerulean | G protein alpha-s internally tagged with mCerulean and EE epitope |
| 66957 | Beta-2-adrenergic receptor-mCerulean | Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus |
| 66968 | G protein alpha-s-mCherry | G protein alpha-s internally tagged with mCherry and EE epitope |
| 67145 | Beta-2-adrenergic receptor-mRFP | Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus |
| 67146 | Beta-2-adrenergic receptor-YFP | Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus |