Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR).
Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
55790 | pENTR-THT | GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance |
55791 | pENTR-THT III | GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance |
55820 | pLIB-TetR-KRAB-IRES-BLAS | retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance |
58242 | pGLTR-FP-GFP | GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker |
58244 | pGLTR-S-PURO | GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance |
58245 | pGLTR-X-GFP | GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP |
58246 | pGLTR-X-PURO | GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection |