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Development of a Multipurpose GATEWAY-Based Lentiviral Tetracycline-Regulated Conditional RNAi System (GLTR).

Sigl R, Ploner C, Shivalingaiah G, Kofler R, Geley S
PLoS One. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764. eCollection 2014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
55790pENTR-THTGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance
55791pENTR-THT IIIGATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance
55820pLIB-TetR-KRAB-IRES-BLASretroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance
58242pGLTR-FP-GFPGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP marker
58244pGLTR-S-PUROGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
58245pGLTR-X-GFPGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP
58246pGLTR-X-PUROGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

Antibodies from Article