Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response.
Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ
Integr Biol (Camb). 2014 May 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55765 | pTorPE-Y-GECO1 | Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells |
| 55766 | CMV-Y-GECO1 | Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells |
| 55767 | AAV-Y-GECO1 | Express Y-GECO1 in neurons |
| 58200 | AAV-hSyn-Lyn-Y-GECO1 | Express the membrane targeted Y-GECO1 in neurons |