Live cell analysis of G protein beta5 complex formation, function, and targeting.

Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
55707	Cer(1-158)-beta-1 in pcDNAI/Amp	An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.
55708	Cer(1-158)-beta-5 in pcDNAI/Amp	An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
55760	Cer(1-158)-RGS7	An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
55761	CFP(1-158)-RGS7t	An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
55762	YFP(1-158)-gamma-2 in pcDNAI/Amp	An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55763	CFP(159-238)-beta-5 in pcDNAI/Amp	A carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.
55776	G protein alpha-o-YFP	This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
55777	CFP(159-238)-gamma-2 in pcDNAI/Amp	A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.
55778	Cer(159-238)-beta-5 in pcDNAI/Amp	A carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.
55779	mCherry-Mem	This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.
55780	G protein alpha-o-R179C-EE	This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
55781	G protein alpha-s-YFP	This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
55782	G protein alpha-q-YFP	This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
60898	Cerulean(159-238) in pcDNAI/Amp	This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.