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RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms.

Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, Diefenbacher M, Stamp G, Downward J
Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
55653HRASexpression of mouse HRAS
55654NRASexpression of mouse NRAS
55655KRAS2Bexpression of mouse KRAS2B
55656ERASexpression of mouse ERAS
55657RALAexpression of mouse RALA
55658RALBexpression of mouse RALB
55659RRAS1expression of mouse RRAS1
55660RRAS2expression of mouse RRAS2
55661MRASexpression of mouse MRAS
55662RIT1expression of mouse RIT1
55663RIT2expression of mouse RIT2
55664RAP1Aexpression of mouse RAP1A
55665RAP1Bexpression of mouse RAP1B
55666RAP2Aexpression of mouse RAP2A
55667RAP2Bexpression of mouse RAP2B
55668RAP2Cexpression of mouse RAP2C
55669DIRAS1expression of mouse DIRAS1
55670DIRAS2expression of mouse DIRAS2
55671RASD1expression of mouse RASD1
55672RASD2expression of mouse RASD2
55673RASL10Bexpression of mouse RASL10B
55674RASL10Aexpression of mouse RASL10A
55675NKIRAS1expression of mouse NKIRAS1
55676NKIRAS2expression of mouse NKIRAS2
55677RERGexpression of mouse RERG
55678RASL11Bexpression of mouse RASL11B
55679RASL12expression of mouse RASL12
55680GEMexpression of mouse GEM
55681REM1expression of mouse REM1
55682REM2expression of mouse REM2
55683RHEBexpression of mouse RHEB
55684RHEBL1expression of mouse RHEBL1
55685RASL11Aexpression of mouse RASL11A
55686DIRAS3expression of human DIRAS3
55692Rac1Expression of mouse Rac1
55693CDC42expression of mouse CDC42
55694RhoGexpression of mouse RhoG
55695RhoQexpression of mouse RhoQ
55696RhoJexpression of mouse RhoJ
55697RhoHexpression of mouse RhoH
55698RhoUexpression of mouse RhoU
55699RhoAexpression of mouse RhoA
55700RhoDexpression of mouse RhoD
55701RND1expression of mouse RND1
55702RhoCexpression of mouse RhoC
55709pSG5-p110α-WTeukaryotic expression of Flag tagged p110a WT
55713pSG5-Myr-FLAG-p110α-DMeukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A
55719pSG5-Myr-FLAG-p110β-DMeukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A
55720pSG5-(nic)p85eukaryotic expression of both SH2 and the inter-SH2 domain of murine p85α.
55722pBacPAK p110a WTHis3 GST tagged WT p110 alpha
55723pBacPAK p110a RBD DMHis3 GST tagged RBD double mutant p110 alpha T207D, K227A
55724pBacPAK p110b WTHis3 GST tagged WT p110 beta

Antibodies from Article