Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 and the Ubiquitin Ligase HERC2.
Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55613 | pcDNA5 FRT/TO SF-HERC2 (ShB-R) | Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells |
| 55614 | pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R) | Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells |
| 55784 | pcDNA SF-HERC2 F1 | Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa) |
| 55785 | pcDNA SF-HERC2 F2 | Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa) |
| 55786 | pcDNA SF-HERC2 F3 | Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa) |
| 55787 | pcDNA SF-HERC2 F4 | Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa) |
| 55788 | pcDNA SF-HERC2 F5 | Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa) |
| 55789 | pcDNA SF-HERC2 F6 | Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa) |