Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics.
Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW
J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 10.1523/JNEUROSCI.3309-13.2014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 57683 | tdEos-Tubulin-C-18 | Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581 |
| 89318 | pCMV-Tag/C448Y with 5'UTR | expression of C448Y spastin M1 and M87 in mammalian cells |
| 89319 | pCMV-Tag/M1C448Y | expression of M1 C448Y spastin in mammalian cells |
| 89320 | pCMV-Tag/M87 C448Y | expression of M87 C448Y spastin in mammalian cells |
| 89321 | pCMV-Tag/WT M1 | expression of M1 C448Y spastin in mammalian cells |
| 89322 | pCMV-Tag/WT M87 | expression of WT M87 spastin in mammalian cells |
| 89323 | pIRES RED/SP M1C448Y | Expression of RFP and M1C448Y spastin in mammalian cells |
| 89324 | pIRES RED/SP M87C448Y | Expression of RFP and M87C448Y spastin in mammalian cells |
| 89325 | pTRE/SP C448Y with 5'UTR | Inducible expression of C448Y spastin M1 and M87 in mammalian cells |
| 89326 | pTRE/SP M1C448Y | Inducible expression of M1C448Y spastin in mammalian cells |
| 89327 | pTRE/SP M1WT | Inducible expression of WT M1 spastin in mammalian cells |
| 89328 | pTRE/SP M87 WT | Inducible expression of WT M87 spastin in mammalian cells |
| 89329 | pTRE/SP M87C448Y | Inducible expression of M87 C448Y spastin in mammalian cells |
| 89330 | pTRE/WT SP with 5'UTR | Inducible expression of WT spastin M1 and M87 in mammalian cells |