Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55195 | CMVp-dCas9-3xNLS-VP64 (Construct 1) | Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector. |
| 55196 | PGK1p-Csy4-pA (Construct 2) | Expresses Csy4 in Mammalian cells for site-specific RNA processing |
| 55197 | P1-EYFP-pA (Construct 5) | Synthetic promoter responsive to gRNA1 |
| 55198 | P2-ECFP-pA (Construct 6) | Synthetic promoter responsive to gRNA2 |
| 55199 | CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22) | CFP reporter along with FF4 miRNA binding sites and 28 nt sequences |
| 55200 | CMVp-dsRed2-Triplex-28-gRNA1-28-pA | Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues. |
| 55201 | CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA | Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues. |
| 55202 | CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA | Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues. |