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Addgene

Michael Davidson Lab: Michael Davidson Empty Backbones

Davidson Lab
unpublished

Plasmids from Article

ID Plasmid Purpose
54491mCherry-Lifeact-7Localization: Action, Excitation: 587, Emission: 610
54492PA-mCherry1-Lifeact-7Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
54493DsRed2-N1Localization: N1 Cloning Vector, Excitation: 558, Emission: 583
54495PA-mCherry1-C1Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
54496mTagBFP-Lifeact-7Localization: Actin, Excitation: 399, Emission: 456
54497dEos-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54502mDsRed-C1Localization: C1 Cloning Vector, Excitation: 557, Emission: 585
54503DsRed2-Peroxisomes-4Localization: Peroxisomes, Excitation: 558, Emission: 583
54507PA-mCherry1-N1Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
54509mPlum-Peroxisomes-2Localization: Peroxisomes, Excitation: 590, Emission: 649
54517mCherry2-N1Localization: N1 Cloning Vector, Excitation: 587, Emission: 610
54520mCherry-Peroxisomes-2Localization: Peroxisomes, Excitation: 587, Emission: 610
54522mPA-GFP-C1Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
54526mGeos2-VESS-Lifeact-7Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
54527tdEos-Lifeact-7Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
54534mKOet-Peroxisomes-2Localization: Peroxisomes, Excitation: 548, Emission: 561
54537Clover2-N1Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
54541mGeos2-VEL-C1Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54542EBFP2-pBADLocalization: Protein Expression Vector , Excitation: 383, Emission: 448
54543mApple-Display-5Localization: Cytoplasm, Excitation: 568, Emission: 592
54544tdEos-Peroxisomes-2Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581
54546mKeima-Red-C1Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
54547wtKikGR-C1Localization: C1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
54548ECFP-Peroxisomes-0Localization: Peroxisomes, Excitation: 433, Emission: 475
54552mRuby-C1Localization: C1 Cloning Vector, Excitation: 558, Emission: 605
54554mMaroon-N1Localization: N1 Cloning Vector, Excitation: 610, Emission: 652
54556mCerulean-Nucleus-12Localization: Nucleus, Excitation: 433, Emission: 475
54558mGeos2-VESS-C1Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54559EBFP2-Lifeact-7Localization: Actin, Excitation: 383, Emission: 448
54560mRuby-Lifeact-7Localization: Actin, Excitation: 558, Emission: 605
54563mCherry2-C1Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
54574mPA-Emerald-N1Localization: N1 Cloning Vector, Excitation: 400 / 487, Emission: 509
54576Clover2-pBADLocalization: Protein Expression Vector , Excitation: 505, Emission: 515
54578PA-sfGFP-C1Localization: C1 Cloning Vector, Excitation: 400 / 485, Emission: 468 / 511
54580mDsRed-N1Localization: N1 Cloning Vector, Excitation: 557, Emission: 585
54581mRuby-N1Localization: N1 Cloning Vector, Excitation: 558, Emission: 605
54586mTagRFP-T-Lifeact-7Localization: Actin, Excitation: 555, Emission: 584
54588Emerald-N1Localization: N1 Cloning Vector, Excitation: 487, Emission: 509
54589mGeos2-VESS-N1Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54591iLOV-C1Localization: C1 Cloning Vector, Excitation: 450, Emission: 495
54595EBFP2-N1Localization: N1 Cloning Vector, Excitation: 383, Emission: 448
54597mKeima-Red-N1Localization: N1 Cloning Vector, Excitation: 440, Emission: 620
54598eqFP611-N1Localization: N1 Cloning Vector, Excitation: 559, Emission: 611
54600Phamret-Peroxisomes-2Localization: Peroxisomes, Excitation: 458 / 475, Emission: 458 / 517
54601sfGFP-Peroxisomes-2Localization: Peroxisomes, Excitation: 485, Emission: 507
54607AcGFP1-C1Localization: C1 Cloning Vector, Excitation: 475, Emission: 505
54608DsRed2-pBADLocalization: Protein Expression Vector , Excitation: 558, Emission: 583
54610mEGFP-Lifeact-7Localization: Actin, Excitation: 488, Emission: 507
54611DsRed2-C1Localization: C1 Cloning Vector, Excitation: 558, Emission: 583
54616AmCyan1-N1Localization: N1 Cloning Vector, Excitation: 453, Emission: 486
54618dKeima-Red-N1Localization: N1 Cloning Vector, Excitation: 440, Emission: 616
54622mEGFP-pBADLocalization: Protein Expression Vector , Excitation: 488, Emission: 507
54623Topaz-pBADLocalization: Protein Expression Vector , Excitation: 514, Emission: 527
54624Dendra1-N1Localization: N1 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
54627mApple-Peroxisomes-2Localization: Peroxisomes, Excitation: 568, Emission: 592
54628mPA-GFP-Peroxisomes-2Localization: Peroxisomes, Excitation: 400 / 504, Emission: 515 / 517
54633mKOet-Lifeact-7Localization: Actin, Excitation: 548, Emission: 561
54634tdEos-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54639mWasabi-Peroxisomes-2Localization: Peroxisomes, Excitation: 493, Emission: 509
54641Phamret-N1Localization: N1 Cloning Vector, Excitation: 458 / 475, Emission: 458 / 517
54646tdEos-C1Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54647mEos-A69V-C1Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54652Phamret-C1Localization: C1 Cloning Vector, Excitation: 458 / 475, Emission: 458 / 517
54653tdTomato-C1Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
54655Dronpa3-C1Localization: C1 Cloning Vector, Excitation: 503, Emission: 518
54659mRaspberry-C1Localization: C1 Cloning Vector, Excitation: 598, Emission: 625
54661mTopaz-Lifeact-7Localization: Actin, Excitation: 514, Emission: 527
54665EBFP2-C1Localization: C1 Cloning Vector, Excitation: 383, Emission: 448
54669mKOet-N1Localization: N1 Cloning Vector, Excitation: 548, Emission: 561
54670mKOet-C1Localization: C1 Cloning Vector, Excitation: 548, Emission: 561
54671mPA-GFP-Lifeact-7Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
54672mCitrine-Peroxisomes-2Localization: Peroxisomes, Excitation: 516, Emission: 529
54673iLOV-N1Localization: N1 Cloning Vector, Excitation: 450, Emission: 495
54675mCrimson3-N1Localization: N1 Cloning Vector, Excitation: 588, Emission: 615
54676mCrimson2-N1Localization: N1 Cloning Vector, Excitation: 588, Emission: 615
54679mPlum-Lifeact-7Localization: Actin, Excitation: 590, Emission: 649
54681wtEos-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54682Dronpa3-N1Localization: N1 Cloning Vector, Excitation: 503, Emission: 518
54687mPA-Emerald-C1Localization: C1 Cloning Vector, Excitation: 400 / 487, Emission: 509
54688mVenus-Lifeact-7Localization: Actin, Excitation: 515, Emission: 528
54689Dendra1-C1Localization: C1 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
54690Dronpa-Peroxisomes-2Localization: Peroxisomes, Excitation: 503, Emission: 518
54691mCerulean-Peroxisomes-2Localization: Peroxisomes, Excitation: 433, Emission: 475
54692mVenus-Peroxisomes-2Localization: Peroxisomes, Excitation: 515, Emission: 528
54694Dendra2-Lifeact-7Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573
54695PS-CFP2-Lifeact-7Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511
54696mEos3.2-Lifeact-7Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
54697mKate-Lifeact-7Localization: Actin, Excitation: 588, Emission: 635
54701ZsYellow1-N1Localization: N1 Cloning Vector, Excitation: 529, Emission: 539
54702ZsGreen1-N1Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
54703AsRed2-N1Localization: N1 Cloning Vector, Excitation: 576, Emission: 592
54705AcGFP1-N1Localization: N1 Cloning Vector, Excitation: 475, Emission: 505
54709mTopaz-C1Localization: C1 Cloning Vector, Excitation: 514, Emission: 527
54711Clover2-C1Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
54712mPA-GFP-N1Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
54713mCerulean-Lifeact-7Localization: Actin, Excitation: 433, Emission: 475
54717mTopaz-N1Localization: N1 Cloning Vector, Excitation: 514, Emission: 527
54718wtKikGR-N1Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
54724Dendra1-C2Localization: C2 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573
54725Kaede-C2Localization: C2 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580
54729mCrimson-N1Localization: N1 Cloning Vector, Excitation: 588, Emission: 615
54731mKO2-Lifeact-7Localization: Actin, Excitation: 551, Emission: 565
54733mCitrine-Lifeact-7Localization: Actin, Excitation: 516, Emission: 529
54734Emerald-C1Localization: C1 Cloning Vector, Excitation: 487, Emission: 509
54736PA-sfGFP-N1Localization: N1 Cloning Vector, Excitation: 400 / 485, Emission: 468 / 511
54738EBFP-C1Localization: C1 Cloning Vector, Excitation: 387, Emission: 450
54739sfGFP-Lifeact-7Localization: Actin, Excitation: 485, Emission: 507
54741mGeos2-VEL-N1Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54744Topaz-C1Localization: C1 Cloning Vector, Excitation: 514, Emission: 527
54746iLOV-Lifeact-7Localization: Actin, Excitation: 450, Emission: 495
54747mApple-Lifeact-7Localization: Actin, Excitation: 568, Emission: 592
54748mOrange2-Lifeact-7Localization: Actin, Excitation: 549, Emission: 565
54751mOrange-pBADLocalization: Protein Expression Vector , Excitation: 548, Emission: 562
54752MiCy-N1Localization: N1 Cloning Vector, Excitation: 472, Emission: 495
54753mGeos-M-N1Localization: N1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54754mEos-A69V-N1Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
54756mKikGR-N1Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593
54759mEGFP-C1Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
54762EGFP-pBADLocalization: Protein Expression Vector , Excitation: 488, Emission: 507
54763mRuby-pBADLocalization: Protein Expression Vector , Excitation: 558, Emission: 605
54766Topaz-N1Localization: N1 Cloning Vector, Excitation: 514, Emission: 527
54767mEGFP-N1Localization: N1 Cloning Vector, Excitation: 488, Emission: 507
54769mKO2-Peroxisomes-2Localization: Peroxisomes, Excitation: 551, Emission: 565
54776Emerald-pBADLocalization: Protein Expression Vector , Excitation: 487, Emission: 509
54788JRed-C1Localization: C1 Cloning Vector, Excitation: 584, Emission: 610
54789JRed-N1Localization: N1 Cloning Vector, Excitation: 584, Emission: 610
54791Kindling-N1Localization: N1 Cloning Vector, Excitation: 580, Emission: 515
54805mClover-pBADLocalization: Protein Expression Vector , Excitation: 505, Emission: 515
54806mCrimson-C1 Localization: C1 Cloning Vector, Excitation: 588, Emission: 615
54807mCrimson2-C1 Localization: C1 Cloning Vector, Excitation: 588, Emission: 615
54808mCrimson3-C1 Localization: C1 Cloning Vector, Excitation: 588, Emission: 615
54815mGeos-M-C1Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
54816mGeos-M-Lifeact-7Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
54817mGeos2-VEL-Lifeact-7Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
54818mGeos2-VEMS-Lifeact-7Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581
54830mKind1-C1Localization: C1 Cloning Vector, Excitation: 580, Emission: 515
54831mKind1-N1Localization: N1 Cloning Vector, Excitation: 580, Emission: 515
54832mKind2-C1Localization: C1 Cloning Vector, Excitation: 580, Emission: 515
54833mKind2-N1Localization: N1 Cloning Vector, Excitation: 580, Emission: 515
54835mMaroon-C1 Localization: C1 Cloning Vector, Excitation: 610, Emission: 652
54836mNeptune2-C1Localization: C1 Cloning Vector, Excitation: 600, Emission: 650
54837mNeptune2-N1Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
54840mRuby-Peroxisomes-2Localization: Peroxisomes, Excitation: 558, Emission: 605
54841mTopaz-pBADLocalization: Protein Expression Vector , Excitation: 514, Emission: 527
54844mTurquoise2-pBADLocalization: Protein Expression Vector , Excitation: 434, Emission: 474
54850rsEmerald-pBADLocalization: Protein Expression Vector , Excitation: 491, Emission: 508
54851rsGFP1-pBADLocalization: Protein Expression Vector , Excitation: 493, Emission: 510
54853SuperSOG-C1Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528
55046mKusabira Orange-N1Empty backbone for fusing gene of interest to N-term of mKO

Antibodies from Article