Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting.

Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH
J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
54468	YFP(1-158)-beta-1 in pcDNAI/Amp	An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
54469	YFP(1-158)-beta-2 in pcDNAI/Amp	An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
54470	YFP(1-158)-beta-5 in pcDNAI/Amp	An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
54471	YFP(159-238)-gamma-1 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
54472	YFP(159-238)-gamma-2 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
54473	YFP(159-238)-gamma-7 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
60888	YFP(1-158) in pcDNAI/Amp	This vector attaches YFP(1-158) to the N-terminus of a protein.
60889	YFP(159-238) in pcDNAI/Amp	This vector attaches YFP(159-238) to the N-terminus of a protein.