Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits.

Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
55191	YFP(159-238)-gamma-5 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-5. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55192	YFP(159-238)-gamma-10 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-10. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55193	YFP(159-238)-gamma-11 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55194	YFP(159-238)-gamma-12 in pcDNAI/Amp	A carboxyl-terminal YFP fragment was fused to Ggamma-12. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55592	CFP(159-238)-beta-1 in pcDNAI/Amp	A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
55615	CFP(1-158)-gamma-1 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55616	CFP(1-158)-gamma-2 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55617	CFP(1-158)-gamma-5 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55618	CFP(1-158)-gamma-7 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55619	CFP(1-158)-gamma-10 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55620	CFP(1-158)-gamma-11 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55621	CFP(1-158)-gamma-12 in pcDNAI/Amp	An amino-terminal CFP fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55622	mRFP-mem	This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.
55623	Cer(1-158)-gamma-1 in pcDNAI/Amp	An amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55624	Cer(1-158)-gamma-2 in pcDNAI/Amp	An amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55625	Cer(1-158)-gamma-5 in pcDNAI/Amp	An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55626	Cer(1-158)-gamma-7 in pcDNAI/Amp	An amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55627	Cer(1-158)-gamma-10 in pcDNAI/Amp	An N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55628	Cer(1-158)-gamma-11 in pcDNAI/Amp	An N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
55629	Cer(1-158)-gamma-12 in pcDNAI/Amp	An N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
60895	CFP(1-158) in pcDNAI/Amp	This vector attaches CFP(1-158) to the N-terminus of a protein.
60896	CFP(159-238) in pcDNAI/Amp	This vector attaches CFP(159-238) to the N-terminus of a protein.
60897	Cerulean(1-158) in pcDNAI/Amp	This vector attaches Cerulean(1-158) to the N-terminus of a protein.