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Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload.

Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM
J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
54163mEmerald-MCM2-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54164mEmerald-MCM2-N-22Localization: Nucleus, Excitation: 487, Emission: 509
54165mEmerald-MCM3-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54166mEmerald-MCM3-N-22Localization: Nucleus, Excitation: 487, Emission: 509
54167mEmerald-MCM4-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54168mEmerald-MCM4-N-22Localization: Nucleus, Excitation: 487, Emission: 509
54169mEmerald-MCM5-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54170mEmerald-MCM5-N-22Localization: Nucleus, Excitation: 487, Emission: 509
54171mEmerald-MCM6-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54172mEmerald-MCM6-N-22Localization: Nucleus, Excitation: 487, Emission: 509
54173mEmerald-MCM7-C-19Localization: Nucleus, Excitation: 487, Emission: 509
54174mEmerald-MCM7-N-22Localization: Nucleus, Excitation: 487, Emission: 509
55056mCherry-H2B-6 Localization: Nucleus/Histones, Excitation: 587, Emission: 610

Antibodies from Article