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Fluorescent protein tracking and detection: fluorescent protein structure and color variants.

Rizzo MA, Davidson MW, Piston DW
Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
54145mEmerald-LC-Myosin-C-10Localization: Light Chain Myosin, Excitation: 487, Emission: 509
54305mEmerald-Vinexin-C-14Localization: Focal Adhesions, Excitation: 487, Emission: 509
54501EGFP-Peroxisomes-0Localization: Peroxisomes, Excitation: 488, Emission: 507
54596mOrange2-Peroxisomes-2Localization: Peroxisomes, Excitation: 549, Emission: 565
54867mApple-Annexin(A4)-12
54940mApple-Profilin-10Localization: Cytoskeleton, Excitation: 568, Emission: 592
54958mApple-VASP-5Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592
54967mCherry-Actin-C-18Localization: Actin, Excitation: 587, Emission: 610
55019mCherry-Clathrin LC-15Localization: Clathrin Vesicles, Excitation: 587, Emission: 610
55157mCherry-Vimentin-C-18Localization: Intermediate Filaments, Excitation: 587, Emission: 610
55263mTagBFP-Lysosomes-20Localization: Lysosome Membrane, Excitation: 399, Emission: 456
55342ECFP-ER-0Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475
55379mCerulean-Keratin-17Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475
55380mCerulean-LaminB1-10Localization: Nuclear Envelope, Excitation: 433, Emission: 475
55398mCerulean-Zyxin-6Localization: Focal Adhesions, Excitation: 433, Emission: 475
55971mPlum-Gamma-Tubulin-17Localization: Centrosomes, Excitation: 590, Emission: 649
56325mVenus-CENPB-N-22Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528
56421EGFP-Actin-7Localization: Actin, Excitation: 488, Emission: 507
56433EGFP-Endosomes-13Localization: Endosomes, Excitation: 488, Emission: 507
56450EGFP-Tubulin-6Localization: Microtubules, Excitation: 488, Emission: 507
56508mWasabi-Mito-7Localization: Mitochondria, Excitation: 493, Emission: 509
56584EYFP-Clathrin-19Localization: Clathrin Vesicles, Excitation: 513, Emission: 527
56596EYFP-Mito-7Localization: Mitochondria, Excitation: 513, Emission: 527
57690tdEos-Vimentin-N-18Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581
57842mKO-Fibrillarin-7Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561
57848mKO-Mito-7Localization: Mitochondria, Excitation: 548, Emission: 561
58014mTagRFP-T-Endosomes-14Localization: Endosomes, Excitation: 555, Emission: 584

Antibodies from Article