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Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms.

Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J
Nat Methods. 2013 Jul;10(7):653-8. doi: 10.1038/nmeth.2488. Epub 2013 May 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
54075mEmerald-EB3-7Localization: MT End Binding Protein, Excitation: 487, Emission: 509
57452mEos3.2-Clathrin-15Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission: 516 / 580
57454mEos3.2-EB3-7Localization: MT End Binding Protein, Excitation: 507 / 572, Emission: 516 / 580
57658tdEos-PMP-N-10Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581

Antibodies from Article