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TRIM65 regulates microRNA activity by ubiquitination of TNRC6.

Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME
Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
53966pCMVhTRIM65_3FlagHuman TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.
53968pCMVhPOP1_3FlagMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.
53974pCMV3FLAG_hGTPBP4Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.
54320hTRIM65_EGFP_N1Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.
165463pEXPqcxip-hFTSJ3-FLAGHuman FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
165464pCMV3Tag8li-hRBM34-HAHuman RBM34 mammalian expression vector with C-terminal HA tag.
165465pCMV3Tag8li-hDDX50-HAHuman DDX50 mammalian expression vector with C-terminal HA tag.
165466pCMV3Tag8li-hSAPS1-HAHuman SAPS1 mammalian expression vector with C-terminal HA tag.
165468pCMV3Tag8li-hHP1BP3-HAHuman HP1BP3 mammalian expression vector with C-terminal HA tag.
165469pCMV3Tag8li-hDDX41-HAHuman DDX41 mammalian expression vector with C-terminal HA tag.
165470pCMV3Tag8li-hADARB2-3xFLAGHuman ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Antibodies from Article