In toto imaging of embryogenesis with confocal time-lapse microscopy.
Megason SG
Methods Mol Biol. 2009;546:317-32. doi: 10.1007/978-1-60327-977-2_19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53744 | pCS-H2B-EGFP | Contains human Histone H2B fused to EGFP in the pCS2+ expression vector |
| 53745 | pCS-H2B-mRFP1 | Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector |
| 53746 | pCS-H2B-EYFP | contains fusion of human histone H2B to EYFP in pCS2+ vector. |
| 53748 | pCS-H2B-cerulean | Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector. |
| 53749 | pCS-membrane-ceruleanFP | Contains membrane localized cerulean fluorescent protein in pCS expression vector. |
| 53750 | pCS-memb-mCherry | Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector. |
| 53752 | pCS-H2B-citrine | Contains fusion of human Histone H2B to citrine. |