Activation of prokaryotic transcription through arbitrary protein-protein contacts.
Dove SL, Joung JK, Hochschild A
Nature. 1997 Apr 10;386(6625):627-30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53730 | pACλCI | PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733. |
| 53731 | pBRα | Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734. |
| 53732 | pBRα-σ70 D581G | Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734. |
| 53733 | pACλCI-β-flap (831-1057) | Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest. |
| 53734 | pBRα-β flap (831-1057) | Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest. |
| 53735 | FW102 OL2-62 | Bacterial-two hybrid reporter strain |