Targeted genome modification of crop plants using a CRISPR-Cas system.
Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C
Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
53062 | pU6-gRNA | Transcription of guide RNA (gRNA) in wheat cells |
53063 | pU3-gRNA | Transcription of guide RNA (gRNA) in rice cells |
53064 | pJIT163-2NLSCas9 | Expression of rice codon-optimized Cas9 in plant cells |