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Purification of recombinant high molecular weight two-partner secretion proteins from Escherichia coli.

Fleckenstein JM, Roy K
Nat Protoc. 2009;4(7):1083-92. doi: 10.1038/nprot.2009.87. Epub 2009 Jul 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
53532pJL017This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene
53533pJL030supplies EtpC required for optimal EtpA expression on compatible plasmid

Antibodies from Article