Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.
Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K
Nucleic Acids Res. 2014 Apr 19.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52522 | pRB14 | expresses a myc-tagged version of hCas9 in Drosophila |
| 52527 | pRB17 | serves as template for PCR-mediated fusion of U6-promoter with sgRNA |
| 52528 | pMH3 | serves as PCR-template to generate HR donors for C-terminal GFP-tag |
| 52529 | pMH4 | serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag |
| 52530 | pIW1 | serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag |
| 52531 | pMH5 | expresses FLP recombinase in Drosophila |
| 52532 | pKF251 | Double-GFP reporter for DNA cleavage |
| 52533 | pKF257 | expresses I-SceI nuclease in Drosophila |
| 52559 | pRB20 | serves as PCR-template to generate HR donors for C-terminal YFP-tag |