Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.

Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K
Nucleic Acids Res. 2014 Apr 19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
52522	pRB14	expresses a myc-tagged version of hCas9 in Drosophila
52527	pRB17	serves as template for PCR-mediated fusion of U6-promoter with sgRNA
52528	pMH3	serves as PCR-template to generate HR donors for C-terminal GFP-tag
52529	pMH4	serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag
52530	pIW1	serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag
52531	pMH5	expresses FLP recombinase in Drosophila
52532	pKF251	Double-GFP reporter for DNA cleavage
52533	pKF257	expresses I-SceI nuclease in Drosophila
52559	pRB20	serves as PCR-template to generate HR donors for C-terminal YFP-tag