Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
Daringer NM, Dudek RM, Schwarz KA, Leonard JN
ACS Synth Biol. 2014 Mar 11.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58854 | tTA_BFP | Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901) |
| 58855 | pBI-MCS-EYFP | The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP |
| 58856 | mCherry_LD 0_CS G_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58868 | mCherry_LD 0_TEV | MESA protease chain with mCherry ectodomain and no linker domain |
| 58869 | CD4_LD 0_CS G_tTA-BFP | MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58870 | CD4_LD 0_TEV | MESA protease chain with CD4 ectodomain and no linker domain |
| 58871 | dTomato_LD 0_CS G_tTA-BFP | MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58872 | dTomato_LD 0_TEV | MESA protease chain with dTomato ectodomain and no linker domain |
| 58873 | mCherry_LD 0_CS A_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion |
| 58874 | mCherry_LD 0_CS M_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion |
| 58875 | dTomato_LD 0_CS M_tTA-BFP | MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion |
| 58876 | FRB_LD 0_CS G_tTA-BFP | MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58877 | FKBP_LD 0_TEV | MESA protease chain with Rapamycin FKBP ectodomain and no linker domain |
| 58878 | FKBP_SCF2_LD6_NTEV | MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease |
| 58879 | FRB_SCF2_LD6_CTEV | MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease |