Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.
Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68311 | pENTR5'-attP (pCM254) | Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis. |
| 68313 | pDestattB (pCM268) | Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish |
| 68339 | pDestattB_ubi:EGFP (pCM272) | Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP. |
| 68341 | pDestattB/CY (pCM327) | Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone. |