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High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells.

Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
Nature. 2014 Apr 9. doi: 10.1038/nature13166. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
53118pLenti-OC-IRES-BSDTo create stable cell clones with high-level expression of Cas9 and OCT1
53121pLenti-sgRNA-LibsgRNA expression construction in a 3rd generation lentiviral backbone

Antibodies from Article