High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells.
Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
Nature. 2014 Apr 9. doi: 10.1038/nature13166.
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PubMed
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
53118 | pLenti-OC-IRES-BSD | To create stable cell clones with high-level expression of Cas9 and OCT1 |
53121 | pLenti-sgRNA-Lib | sgRNA expression construction in a 3rd generation lentiviral backbone |