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Disruption of small RNA signaling caused by competition for Hfq.

Hussein R, Lim HN
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
30006HL 818 (=HL 716 + pHL 98)
30008HL 839 (=HL 716 + pHL 108)
30009HL 849 (=HL 716 + micC knockout_KanR)
30012HL 1178 (=HL 862 + pHL 282)
30014HL 1329 (=HL 865 + pHL 344)
30015HL 1474 (=HL 862 + pHL 404)
30017HL 2300 (=HL 716 + pHL 745)
30019HL 2304 (=HL 716 + pHL 269)
30020HL 2305 (=HL 716 + pHL 765)
30021HL 2307 (=HL 716 + pHL 777)
30022HL 2308 (=HL 716 + pHL 814)
30024HL 2312 (=HL 716 + pHL 787)
30025HL 2314 (=HL 716 + pHL 818)
30028HL 2625 (=HL 716 + pHL 908)
30029HL 2627 (=HL 770 + pHL 908)
30030HL 2729 (=HL 716 + rhyB knockout_KanR)
30031HL 2817 (=HL 716 + pHL 720)
30032HL 2818 (=HL 865 + pHL 489)
30033HL 2839 (=HL 2752 + pHL 720)
30034HL 2869 (=HL 2752 + pHL 991)
30036HL 3076 (=HL 716 + pHL 1069)
30047HL 3151 (=HL 716 + pHL 1037)
30048HL 3152 (=HL 716 + pHL 1056)
30051HL 3186 (=HL 716 + pHL 282)
30059HL 3326 (=HL 716 + pHL 1008)
30060HL 3327 (=HL 716 + pHL 1009)
30063HL 3330 (=HL 770 + pHL 1009)
30065HL 3325 (=HL 770 + rhyB knockout_KanR)
30067HL 3347 (=HL 3338 + pHL 720)
30068HL 3357 (=HL 716 + pHL 1191)
30072HL 3375 (=HL 865 + pHL 1008)
30074HL 3395 (=HL 862 + pHL 1009)
30078HL 3448 (=HL 3425 + pHL 1191)
30079HL 3449 (=HL 3262 + pHL 1179)
30080HL 3450 (=HL 3262 + pHL 1191)
30081HL 3545 (=HL 770 + pHL 1213)
30082HL 3612 (=HL 716 + pHL 841)
30083HL 3619 (=HL 716 + pHL 1228)
30084HL 3722 (=HL 716 + pHL 404)
30085HL 3723 (=HL 716 + pHL 489)
30086HL 3724 (=HL 716 + pHL 991)
30088HL 3858 (=HL 716 + pHL 1304)
30089HL 3859 (=HL 716 + pHL 1305)
30093HL 3904 (=HL 770 + pHL 991)
30094HL 4120 (=HL 716 + pHL 1370)
30096HL 4175 (=HL 716 + pHL 1391)
30097HL 4178 (=HL 862 + pHL 1360)
30098HL 4179 (=HL 865 + pHL 1381)
30099HL 4180 (=HL 2752 + pHL 1358)
30100HL 4202 (=HL 716 + pHL 1389)
30104HL 4212 (=HL 716 + pHL 1414)
30105HL 4218 (=HL 716 + pHL 1411)
52931HL 713MG 1655 + lacIq_KanR inserted into the chromosome at the intS site
52932HL 751MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanR
52933HL 852MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA_KanR
52934HL 1120MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + Δhfq_KanR
52935HL 1179MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + Δhfq_KanR
52936HL 3221MG 1655 + lacIq inserted into the chromosome at the intS site + ΔoxyS_KanR
52937HL 3387MG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq + ΔoxyS_KanR
52989pHL 100pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
52990pHL 762pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
52991pHL 802pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
52992pHL 990pLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfp
52993pHL 1011pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
52994pHL 1019pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp
52995pHL 1020pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp
52996pHL 1022pLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfp
52997pHL 1032pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
52998pHL 1033pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
52999pHL 1035pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
53000pHL 1039pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp
53001pHL 1057pCon:RhyB, pLlacO-1:rpoS::gfp
53002pHL 1070pCon:DsrA, pLlacO-1:sodB::gfp
53003pHL 1073pCon:ompC::mCherry, pLlacO-1:sodB::gfp
53004pHL 1075pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
53006pHL 1148pCon:OxyS, pLlacO-1:sodB::gfp
53007pHL 1158pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
53008pHL 1303pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
53009pHL 1394pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp
53010pHL 1405pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
53011pHL 1410pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
60368HL 716Lim Lab Strain
60369HL 770Lim Lab Strain
60370HL 862Lim Lab Strain
60371HL 865Lim Lab Strain
60372HL 1128Lim Lab Strain
60373HL 1188Lim Lab Strain
60374HL 2752Lim Lab Strain
60376HL 3338Lim Lab Strain

Antibodies from Article