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Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.

Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda SP, Vogt A, Elstner M, Oertner TG, Hegemann P
Science. 2014 Mar 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52494pAAV Syn ChR E90R-D156N-T159C 2A tDimerHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
52495pAAV Syn ChR E90R-T159C 2A tDimer Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
52878pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimerChloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons
85461p-mCherry-N1-ChloCHistorical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
85462p-mCherry-N1-slowChloCHistorical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Antibodies from Article