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Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism.

Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, Hamilton AD, Regan L
J Am Chem Soc. 2005 Jan 12;127(1):146-57. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52732pET11a-link-NGFPIPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay
52733pMRBAD-link-CGFPL-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay
52734pET11a-Z-NGFPIPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay
52735pMRBAD-Z-CGFPL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

Antibodies from Article