Cost-benefit tradeoffs in engineered lac operons.
Eames M, Kortemme T
Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52695 | delta-ZYA | MG1655 with Lac Operon removed from native locus |
| 52696 | KIlac | MG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus |
| 52697 | OpLac1 | KIlac with a codon optimized lacZ using preferred codon at every position |
| 52698 | OpLac1-delta-6 | OpLac1 without the first 6 nucleotides of lacZ |
| 52699 | OpLac2 | KIlac with a codon optimized lacZ using codons for a subset of amino acids read by transfer RNAs that are the most highly charged during amino acid starvation |
| 52700 | OpLac2-delta-6 | OpLac2 without the first 6 nucleotides of lacZ |
| 52701 | Oplac1 (37)-KI | KIlac with first 37 bases from Oplac1 |
| 52702 | Oplac2 (37)-KI | KIlac with first 37 bases from Oplac2 |
| 52703 | KI (37)-Oplac1 | Oplac1 with first 37 bases from KIlac |
| 52704 | KI (37)-Oplac2 | Oplac2 with first 37 bases from KIlac |
| 52705 | degtag | KIlac with lacZ C-terminal sssA degradation tag |
| 52706 | delta-Z | KIlac with lacZ deletion |
| 52707 | delta-ZA | KIlac with lacZ & lacA deletion |
| 52708 | delta-YA | KIlac with lacY and lacA deletion |
| 52709 | S56L | KIlac with S56L mutation in lacY (impairs function) |
| 52710 | G794D | KIlac with G794D mutation in lacZ (impairs function) |
| 52711 | G794A | KIlac with G794A mutation in lacZ (impairs function) |
| 52712 | W999F | KIlac with W999F mutation in lacZ (impairs function) |
| 52713 | W999L | KIlac with W999L mutation in lacZ (impairs function) |
| 52714 | E537Q | KIlac with E537Q mutation in lacZ (impairs function) |