A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans.
Tursun B, Cochella L, Carrera I, Hobert O
PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 2009 Mar 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69316 | pBALU1 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp |
| 69317 | pBALU2 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp |
| 69318 | pBALU3 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp |
| 69319 | pBALU4 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of mChO (mCherry variant) |
| 69321 | pBALU6 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion |
| 69324 | pBALU9 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP |
| 69328 | pBALU12 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP |
| 69334 | pBALU18 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOP |
| 69339 | pBALU-ext | It allows to extend the genomic insert in a fosmid. |