Derivation of novel human ground state naive pluripotent stem cells.

Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
52379	OCT4-GFP-2A-PURO transgenic reporter	BAC recombineering generated construct, used as a reporter for OCT4 expression
52380	delta PE OCT4-GFP-2A-PURO transgenic reporter	BAC recombineering generated construct, used as a reporter for OCT4 expression
52381	delta DE OCT4-GFP-2A-PURO transgenic reporter	BAC recombineering generated construct, used as a reporter for OCT4 expression
52382	mouse deltaPE Oct4-GFP transgenic reporter	BAC recombineering generated construct, used as a reporter for mouse Oct4 expression
52412	FOR human OCT4 TALEN	TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
52413	REV human OCT4 TALEN	TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
52414	pGL3-human OCT4 DE-SV40-Luc	Luciferase reporter plasmid for DE OCT4 enhancer activity
52415	pGL3-human OCT4 PE-SV40-Luc	Luciferase reporter plasmid for PE OCT4 enhancer activity
52419	PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO	Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.
60527	mouse Oct4-GFP GOF18 transgenic reporter	BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)