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Expression and purification of soluble monomeric streptavidin in Escherichia coli.

Demonte D, Dundas CM, Park S
Appl Microbiol Biotechnol. 2014;98(14):6285-95. doi: 10.1007/s00253-014-5682-y. Epub 2014 Apr 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
52319pET-MBP-mSA2Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
52320pET-Trx-mSA2Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Antibodies from Article