N6-methyladenosine-dependent regulation of messenger RNA stability.
Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai Q, Jia G, Ren B, Pan T, He C
Nature. 2014 Jan 2;505(7481):117-20. doi: 10.1038/nature12730. Epub 2013 Nov 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52300 | pcDNA3-flag-YTHDF2 | full length YTHDF2 with N-terminal flag tag for mammalian cell expression |
| 52301 | pGEx-4T-1-YTHDF2 | Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression |
| 52302 | pcDNA3-flag-YTHDF2-N | Flag-tagged YTHDF2 N-terminal domain, made by mutating to E384(GAA) to stop codon(TAA) |
| 52303 | pcDNA3-flag-YTHDF2-C | Flag-tagged YTHDF2 C-terminal domain, from E384 to the end |
| 70087 | pGEx-4T-1-YTHDF1 | Encode YTHDF1 with N-terminal GST tag for E. coli expression |
| 70088 | pGEx-4T-1-YTHDF3 | Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression |