Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA
Mol Cell. 2009 Oct 9;36(1):39-50. doi: 10.1016/j.molcel.2009.09.022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52293 | Split 'n coexpress CUL3-RBX1 | bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1 |
| 52294 | His-MBP human SPOP (aa 28-337) | bacterial expression of human SPOP (aa 28-337) fused to His-MBP |
| 52295 | His Puc (aa 1-393) | Bacterial expression of His tagged Drosophila Puc fragment |