Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.

Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
50915	pHAGE TRE dCas9	Tet-regulatable dCas9 lentiviral expression vector
50916	pHAGE TRE dCas9-VP64	Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector
50917	pHAGE TRE dCas9-KRAB	Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector
50918	pHAGE EF1α dCas9-VP64	Constitutive dCas9-VP64 lentiviral expression vector
50919	pHAGE EF1α dCas9-KRAB	Constitutive dCas9-KRAB 2nd generation lentiviral expression vector
50920	pLKO.1-puro U6 sgRNA BfuAI stuffer	U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites
50921	pLKO.1-puro U6 sgRNA Oct4A -12	U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS
50922	pLKO.1-puro U6 sgRNA Oct4A -158	U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS
50923	pLKO.1-puro U6 sgRNA SOX17 -91	U6 driven sgRNA targeting Sox17 -91 bp from TSS
50924	pLKO.1-puro U6 sgRNA SOX17 -126	U6 driven sgRNA targeting Sox17 -126 bp from TSS
50925	pLKO.1-puro U6 sgRNA SOX17 -177	U6 driven sgRNA targeting Sox17 -177 bp from TSS
50926	pLKO.1-puro U6 sgRNA SOX17 -296	U6 driven sgRNA targeting Sox17 -296 bp from TSS
50927	pLKO.1-puro U6 sgRNA CAG	U6 driven sgRNA negative control