Jesse Rinehart Lab: Rinehart Lab Phosphoprotein synthesis components

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Plasmids from Article

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ID	Plasmid	Purpose
52054	pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)	Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
52053	pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP	Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.