Deterministic direct reprogramming of somatic cells to pluripotency.
Rais Y, Zviran A, Geula S, Gafni O, Chomsky E, Viukov S, Mansour AA, Caspi I, Krupalnik V, Zerbib M, Maza I, Mor N, Baran D, Weinberger L, Jaitin DA, Lara-Astiaso D, Blecher-Gonen R, Shipony Z, Mukamel Z, Hagai T, Gilad S, Amann-Zalcenstein D, Tanay A, Amit I, Novershtern N, Hanna JH
Nature. 2013 Oct 3;502(7469):65-70. doi: 10.1038/nature12587. Epub 2013 Sep 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52356 | FUW-Mbd3 | Lentiviral expression vector for constitutive expression of Mbd3 |
| 52358 | 5' MBD3 TALEN | TALEN vector for targeting human MBD3 locus (5') |
| 52359 | 3' MBD3 TALEN | TALEN vector for targeting human MBD3 locus (3') |
| 52362 | pNTK human MBD3 PGK-Neo-pA flox | Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles. |
| 52371 | FUW-Flag-Mbd3 | Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 |
| 52372 | FUW-Flag-Mbd3 delta1-70 | Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant |
| 52373 | FUW-Flag-Mbd3 delta 25-83 | Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant |
| 52374 | FUW-Flag-Mbd3 delta49-63 | Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant |
| 52376 | pBRPy CAGGS-Mbd3-IRES-PURO | Mammalian expression vector for constitutive expression of Mbd3 |
| 52409 | pBRY-nuclear mCherry-IRES-PURO | Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance. |
| 52416 | FUW-CAGGS-ERAS | Lentiviral vector encoding constitutive expression of human ERAS cDNA |
| 52417 | FUW-TetO-lox-ERAS | Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert |