Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination.
Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, Sandoval-Guzman T, Crawford K, Tanaka EM
Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub 2014 Feb 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51817 | pBSII-SK-mTol2-MCS | To generate transgenic axolotls using Tol2 or SceI mediated transgenics |
| 51818 | T7-TPase | For in vitro transcription of Tol2 transposase using T7 Promoter |