Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion Transport and Transporter Activation Revealed by Internal Crosslinking between Transmembrane Domains 10 and 11/12.
Monette MY, Somasekharan S, Forbush B
J Biol Chem. 2014 Jan 22.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50863 | pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835) | Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50864 | pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841) | Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50865 | pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847) | Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50866 | pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809) | Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50867 | pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810) | Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50868 | pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813) | Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50869 | pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838) | Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50870 | pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816) | Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50873 | pcDNA3.1 Flag YFP hNKCC1 N672C (NT474) | Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50874 | pcDNA3.1 Flag YFP hNKCC1 V673C (NT437) | Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50875 | pcDNA3.1 Flag YFP hNKCC1 I674C (NT475) | Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50876 | pcDNA3.1 Flag YFP hNKCC1 A675C (NT441) | Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50877 | pcDNA3.1 Flag YFP hNKCC1 P676C (NT443) | Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50878 | pcDNA3.1 Flag YFP hNKCC1 I677C (NT467) | Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50879 | pcDNA3.1 Flag YFP hNKCC1 I678C (NT445) | Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50880 | pcDNA3.1 Flag YFP hNKCC1 S679C (NT447) | Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50881 | pcDNA3.1 Flag YFP hNKCC1 N680C (NT468) | Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50884 | pcDNA3.1 Flag YFP hNKCC1 I730C (NT504) | Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50885 | pcDNA3.1 Flag YFP hNKCC1 N731C (NT505) | Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50889 | pcDNA3.1 Flag YFP hNKCC1 A735C (NT509) | Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50890 | pcDNA3.1 Flag YFP hNKCC1 L736C (NT522) | Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50891 | pcDNA3.1 Flag YFP hNKCC1 L737C (NT523) | Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50905 | pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT540) | Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50906 | pcDNA3.1 Flag YFP hNKCC1 C723S C724V F728C (NT542) | Expresses human NKCC1 C723S C724V F728C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50907 | pcDNA3.1 Flag YFP hNKCC1 C723S C724V V729C (NT543) | Expresses human NKCC1 C723S C724V V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50909 | pcDNA3.1 Flag YFP hNKCC1 C723S C724V W733C (NT547) | Expresses human NKCC1 C723S C724V W733C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50910 | pcDNA3.1 Flag YFP hNKCC1 C723S C724V A735C (NT549) | Expresses human NKCC1 C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. |
| 50912 | pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94) | Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells |
| 50913 | pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95) | Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells |
| 50914 | pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96) | Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells |