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Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.

Matsuda T, Miyawaki A, Nagai T
Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 2008 Mar 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
51951mSECFP/pcDNA3mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein
51953Phamret/pcDNA3mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.
51954Phamret-Fibrillarin/pcDNA3mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization
51955Phamret-H2B/pcDNA3mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to H2B

Antibodies from Article