RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli.
Malagon F
RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51557 | pBAD24-fis | E. coli fis ORF cloned into pBAD24 expression vector |
| 51558 | pBAD24-sfGFPx1 | Superfolder GFP ORF cloned into pBAD24 for expression in E. coli |
| 51559 | pBAD24-sfGFPx2 | Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP) |
| 51560 | pBAD24-rnc-sfGFP | Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24). |