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Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes.

Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O
Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
48203Twitch-2B pRSETBFluorescent reporter for calcium signaling
48204Twitch-3 pRSETBFluorescent reporter for calcium signaling
49531Twitch-2B pcDNA3Fluorescent reporter for calcium signaling
49532Twitch-3 pcDNA3Fluorescent reporter for calcium signaling
49533Twitch-4 pcDNA3Fluorescent reporter for calcium signaling
49812Twitch-1 pcDNA3Fluorescent reporter for calcium signaling
100039pAAV.CAG.Flex.Twitch2B.WPRE.SV40AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling
100040pAAV.hSyn1.Twitch2B.WPRE.SV40AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling
104500Twitch-2C pRSETBfluorescent reporter for calcium signaling
104501Twitch-2C pcDNA3fluorescent reporter for calcium signaling

Antibodies from Article