Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling.
Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R
Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51246 | pEF5/FRT/DEST-3xHA/mGli3/Flag | Encodes N-3xHA-TEV-mouseGli3-Flag-C |
| 51247 | pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A | Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala |
| 51248 | pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A | Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala |
| 51249 | pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A | Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala |
| 51250 | pEF5/FRT/DEST-3xHA/mGli2 | Encodes N-3xHA-TEV-mouseGli2-C |
| 51251 | pEF5/FRT/DEST-3xHA/mGli2 P1-6A | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala |
| 51252 | pEF5/FRT/DEST-3xHA/mGli2 P1-4A | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala |
| 51253 | pEF5/FRT/DEST-3xHA/mGli2 P5-6A | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala |
| 51254 | pEF5/FRT/DEST-3xHA/mGli2 P5-6D | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp |
| 51255 | pEF5/FRT/DEST-3xHA/mGli2 Pc-gA | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala |
| 51256 | pEF5/FRT/DEST-3xHA/mGli2 Pc-gE | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu |
| 51257 | pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A | Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala |