Genomic mining of prokaryotic repressors for orthogonal logic gates.
Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
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PubMed
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Article
Deposited by Christopher Voigt's lab, these response function plasmids (pRF-) contain a transcriptional repressor, which controls the expression of a YFP output. Repressors are under the control of the IPTG-inducible Ptac promoter. Expression of the YFP gene is controlled by a synthetic constitutive promoter that contains a repressor operator sequence. The YFP output is repressed in the presence of IPTG. The various circuit plasmids achieve either the NAND (pLux-LmrA and pNAND-PhlF) or AND (pAND) behavior. To generate the NAND behavior, the following input concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 20 µM 3OC6HSL (-/+), and 1 mM IPTG and 20 µM 3OC6HSL (+/+).To generate the AND behavior, the following inducer concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 100 ng/mL aTc (-/+), and 1 mM and 100 ng/mL aTc (+/+).
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49355 | pRF-AmtR | |
| 49356 | pRF-AmeR | |
| 49357 | pRF-BetI | |
| 49358 | pRF-BM3R1 | |
| 49359 | pRF-ButR | |
| 49360 | pRF-HapR | |
| 49361 | pRF-HlyIIR | |
| 49362 | pRF-IcaR | |
| 49363 | pRF-LitR | |
| 49364 | pRF-LmrA | |
| 49365 | pRF-McbR | |
| 49366 | pRF-Orf2 | |
| 49367 | pRF-PhlF | |
| 49368 | pRF-PsrA | |
| 49369 | pRF-QacR | |
| 49370 | pRF-ScbR | |
| 49371 | pRF-SmcR | |
| 49372 | pRF-SrpR | |
| 49373 | pRF-TarA | |
| 49374 | pRF-TetR | |
| 49375 | pLux-LmrA | |
| 49376 | pNAND-PhlF | |
| 49377 | pAND | Expression of AND gate in bacteria |
| 51148 | pET21a-AcrR | |
| 51149 | pET21a-AmeR | |
| 51150 | pET21a-AmrR | |
| 51151 | pET21a-AmtR | |
| 51154 | pET21a-BarA | |
| 51155 | pET21a-BarB | |
| 51156 | pET21a-BetI | |
| 51157 | pET21a-BM1P1 | |
| 51158 | pET21a-BM3R1 | |
| 51159 | pET21a-BpeR | |
| 51161 | pET21a-CalR1 | |
| 51162 | pET21a-CampR | |
| 51163 | pET21a-CasR | |
| 51164 | pET21a-CprB | |
| 51166 | pET21a-Cyp106 | |
| 51167 | pET21a-DhaR | |
| 51168 | pET21a-Ef0113 | |
| 51170 | pET21a-EthR | |
| 51172 | pET21a-HapR | |
| 51173 | pET21a-HemR | |
| 51174 | pET21a-HlyIIR | |
| 51176 | pET21a-IcaR-e | |
| 51177 | pET21a-IfeR | |
| 51178 | pET21a-JadR2 | |
| 51179 | pET21a-KstR | |
| 51181 | pET21a-LitR | |
| 51183 | pET21a-LuxT | |
| 51184 | pET21a-McbR | |
| 51188 | pET21a-MtrR | |
| 51189 | pET21a-NonG | |
| 51191 | pET21a-Orf2 | |
| 51192 | pET21a-orfL6 | |
| 51193 | pET21a-PaaR | |
| 51195 | pET21a-PqrA | |
| 51210 | pET21a-TylP |