Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions.
Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, Ploegh HL
Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub 2013 Aug 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51138 | pET28a-SrtAdelta59 | expression plasmid for N-terminal his tagged S.aureus SrtA delta 59 |
| 51139 | pET28a-SpySrtA | expression plasmid for N-terminal his tagged S.pyogenes SrtA |