Optimisation of the Schizosaccharomyces pombe urg1 Expression System.
Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, Carr AM
PLoS One. 2013 Dec 20;8(12):e83800. doi: 10.1371/journal.pone.0083800.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48975 | pAW8ENdeI | for Cre-lox cassette exchange of untagged gene sequences into the S. pombe urg1 locus |
| 48976 | pAW8ENdeI-3XDSR | for Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus |
| 48978 | pAW8ENdeI-4XDSR | for Cre-lox cassette exchange of untagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus |
| 48980 | pAW8ENdeI-6XDSR | for Cre-lox cassette exchange of untagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus |
| 48981 | pAW8ENdeI-8XDSR | for Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus |
| 48983 | pAW8ENdeI-spo5DSR | for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus |
| 48988 | pAW8ENdeI-cyEGFP | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus |
| 48989 | pAW8ENdeI-c3HA | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus |
| 48990 | pAW8ENdeI-cHAIAA17 | for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus |
| 49863 | pAW8ENdeI-cyEGFP-3XDSR | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus |
| 49864 | pAW8ENdeI-cyEGFP-4XDSR | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus |
| 49865 | pAW8ENdeI-cyEGFP-6XDSR | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus |
| 49866 | pAW8ENdeI-cyEGFP-8XDSR | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus |
| 49867 | pAW8ENdeI-cyEGFP-spo5DSR | for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus |
| 49868 | pAW8ENdeI-c3HA-3XDSR | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus |
| 49869 | pAW8ENdeI-c3HA-4XDSR | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus |
| 49870 | pAW8ENdeI-c3HA-6XDSR | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus |
| 49871 | pAW8ENdeI-c3HA-8XDSR | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus |
| 49872 | pAW8ENdeI-c3HA-spo5DSR | for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus |
| 49873 | pAW8ENdeI-cHAIAA7-spo5DSR | for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus |