Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing.
Gao Y, Zhao Y
J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51055 | pACT2-CAS9c | Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed. |
| 51056 | pRS316-RGR-GFP | Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'. |
| 51057 | pRS316-RGR-GFP-mHH | Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end. |
| 51058 | pRS316-RGR-GFP-mHDV | Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end. |
| 51059 | pRS316-RGR-GFP-mm | Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end. |