Recombinant reconstitution of sumoylation reactions in vitro.
Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53139 | pET23a-His-hRanGAP1tail | E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag |
| 53140 | pGEX3x-hRanBP2-(IR1+M) | E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity |
| 53141 | pET28a-HisYFP-Sp100 | E.coli expression of HisYFP tagged human Sp100 fragment |
| 53142 | pET11a-SUMO2 | E.coli expression of untagged mature human SUMO2 (amino acids 1-92) |
| 53143 | pET11a-SUMO3 | E.coli expression of untagged mature human SUMO3 (amino acids 1-93) |