The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
Cell. 2002 Jan 11;108(1):109-20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53120 | pGEX3X-RanBP2dFG | E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity |
| 53135 | pET28a-His-hAos1 | E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag |
| 53136 | pET11d-hUba2 | E.coli expression of full length untagged human Uba2 (SAE2) |
| 53137 | pET23a-mUbc9 | E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human) |
| 53138 | pET11a-SUMO1 | E.coli expression of untagged mature human SUMO1 (amino acids 1-97) |