Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic lateral sclerosis.
Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F, Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler AD
Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. Epub 2012 Mar 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50733 | pSLIK-NFLAG-hEWSR1WT-CMyc | Produces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G |
| 50734 | pSLIK-NFLAG-hEWSR1G511A-CMyc | Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G |
| 50736 | pSLIK-NFLAG-hEWSR1P552L-CMyc | Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G |
| 84887 | pDONR221-EWSR1 Stop | DONOR vector for Gateway cloning of EWSR1 |
| 84888 | pDONR221-EWSR1 No Stop | DONOR vector for Gateway cloning of EWSR1 No Stop |
| 84890 | pDONR221-EWSR1 g1532c No Stop | DONOR vector for Gateway cloning of EWSR1 g1532c No Stop |
| 84891 | pDONR221-EWSR1 c1655t Stop | DONOR vector for Gateway cloning of EWSR1 c1655t Stop |
| 84892 | pDONR221-EWSR1 c1655t No Stop | DONOR vector for Gateway cloning of EWSR1 c1655t No Stop |
| 84893 | pDONR221-EWSR1 g1750a Stop | DONOR vector for Gateway cloning of EWSR1 g1750a Stop |
| 84894 | pDONR221-EWSR1 g1750a No Stop | DONOR vector for Gateway cloning of EWSR1 g1750a No Stop |