Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.
Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50716 | pCAG-EGxxFP | 5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution. |
| 50717 | pCAG-EGxxFP-Cetn1 | Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution. |
| 50718 | pX330-Cetn1/1 | pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution. |