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Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.

Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T
Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
50664p1HDContains first TALE repeat harboring HD as RVD
50665p1NGContains first TALE repeat harboring NG as RVD
50666p1NIContains first TALE repeat harboring NI as RVD
50667p1NNContains first TALE repeat harboring NN as RVD
50668p2HDContains second TALE repeat harboring HD as RVD
50669p2NGContains second TALE repeat harboring NG as RVD
50670p2NIContains second TALE repeat harboring NI as RVD
50671p2NNContains second TALE repeat harboring NN as RVD
50672p3HDContains third TALE repeat harboring HD as RVD
50673p3NGContains third TALE repeat harboring NG as RVD
50674p3NIContains third TALE repeat harboring NI as RVD
50675p3NNContains third TALE repeat harboring NN as RVD
50676p4HDContains fourth TALE repeat harboring HD as RVD
50677p4NGContains fourth TALE repeat harboring NG as RVD
50678p4NIContains fourth TALE repeat harboring NI as RVD
50679p4NNContains fourth TALE repeat harboring NN as RVD
50680pFUS2_a1aIntermediate array vector
50681pFUS2_a2aIntermediate array vector
50682pFUS2_a2bIntermediate array vector
50683pFUS2_a3aIntermediate array vector
50684pFUS2_a3bIntermediate array vector
50685pFUS2_a4aIntermediate array vector
50686pFUS2_a4bIntermediate array vector
50695pFUS2_b1Intermediate array vector
50696pFUS2_b2Intermediate array vector
50697pFUS2_b3Intermediate array vector
50698pFUS2_b4Intermediate array vector
50699ptCMV-136/63-VR-HDFinal destination vector
50700ptCMV-136/63-VR-NGFinal destination vector
50701ptCMV-136/63-VR-NIFinal destination vector
50702ptCMV-136/63-VR-NNFinal destination vector
50703ptCMV-153/47-VR-HDFinal destination vector
50704ptCMV-153/47-VR-NGFinal destination vector
50705ptCMV-153/47-VR-NIFinal destination vector
50706ptCMV-153/47-VR-NNFinal destination vector

Antibodies from Article